Prof. Liu   
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    Education

    · B.S. Microbial engineering, School of Life Science (2002), Shandong University, China

    · Exchange Ph.D student, Department of Chemistry(2006-2007), Brown University, USA

    · Ph.D. Microbiology(2008), School of Life Science & Biotechnology, Shanghai Jiaotong University, China

     

    Professional Experience

    · Director, Hubei Engineering Laboratory for Synthetic Microbiology, China

    Deputy Chief Engineer, Wuhan Institute of Biotechnology, China (2012- )

    · Professor, Wuhan University School of Pharmaceutical Sciences, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, China (2010.9- )

    · Post-doc researcher, Department of Chemistry, Stanford University, USA (2008-2010)

     

    Special Honors and Awards

    · 2017

     

    · 2016

    · 2014

    Provincial government special allowance; Outstanding young talents, Hubei, China

    Excellent young scientists and technicians, Wuhan, Hubei, China

    National Top-Notch Young Talents Program of National High-level Personnel of Special Support Program,  China

    · 2012

    "Chutian scholar" Professor, Hubei, China

    · 2012

    The National Science Fund for Excellent Young Scholars, China

    · 2010

    New Century Excellent Talents in University, China

     

    Selected Professional Positions

    · 2017

    · 2016

    Biotechnology Journal, Editor on Board

    Current Opinion in Biotechnology special issue "Pharmacetical Biotech 2017"

    Co-editor

    · 2016

    ACS Synthetic Biology, Editor on Board

    · 2016

    Metabolic Engineering, Editor on Board

    · 2016

    Acta microbiologica Sinica, Editor on Board

    · 2016

    Hubei Food And Drug Administration, consultant

    · 2015

    Synthetic and Systems Biotechnology, Editor on Board

    · 2014

    F1000prime, Associate Faculty Member

    · 2013

    Frontiers in Synthetic Biology, Editor on Board

    Selected Research Grants

    · 2017.08-2020.8

     

     

     

    · 2017.01-2020.12

     

    · 2016.01-2018.12

    Natural Science Foundation of Hubei Province of China (No. 2017CFA054) Project Name: Directed synthesis metabolism system guides biological manufacturing industrialization(Principal investigator)

    The National Science Fund for General Program(No. 31670090) Project Name: Highly efficient heterologous synthesis of bactericin based on quantitative metabonomics analysis(Principal investigator)

    National Top-Notch Young Talents Program of National High-level Personnel of Special Support Program (Principal investigator)

    · 2012.10-2016.10 

    The National 973 Program of China Grant (No. 2012CB721001) Project Name:  Screening, modification and standardization of biological parts for synthesis biology (Co-investigator)

    · 2013.01-2015.12

    The National Science Fund for Excellent Young Scholars(No. 31222002) Project Name:  Breeding and Genetics of Applied Microorganisms (Principal investigator)

    · 2011.01-2015.08

    The National 973 Program of China Grant (No. 2011CBA00806) Project Name: Development of bio-based production through synthetic bioengineering (Co-investigator)

     

    Patents

    1. Tiangang Liu, Zhijie Liu, Yixuan Wang, Zixin Deng. “A method of producing 3-hydroxypropionic acid, acrylic acid and propionic acid in the method of fixing carbon dioxide microorganisms”. China Patent 201410035584.6, 24 January 2014
    2. Tiangang Liu, Ran Liu, Aisi Fu. “Method for biologically synthesizing fatty alcohol from fatty acyl ACP (acyl carrier protein) reductase”. China Patent 201210330969.6, 01 January 2014
    3. Tiangang Liu, Fayin Zhu, Xiaoying Cao, Yuchen Zhang, Guangkai Bian, Zixin Deng. “Microbiological process for producing taxadiene and its application”. China Patent 201310209544.8, 22 November 2013
    4. Tiangang Liu, Ran Liu, Fayin Zhu. “Method for directly synthesizing fatty alcohol in heterotrophic microorganisms”. China Patent 201110327887.1, 26 October 2013
    5. Tiangang Liu, Fayin Zhu, Zixin Deng. “Bacterial strain for producing farnesene and its application”. China Patent 201310209421.0, 30 May 2013. This patent has been transfered 500 thousand yuan.
    6. Tiangang Liu, Fayin Zhu, Zixin Deng. “Bacterial strain for producing lycopene and its application”. China Patent 201310209514.3, 30 May 2013
    7. Tiangang Liu, Daoyi Guo. “Microorganisms and their function”. China Patent 201310115925.6, 03 April 2013
    8. Tiangang Liu, Kaiyue Wu. “Process for the production of fatty alkenes and fatty alkanes”. China Patent 201210001015, 04 January 2012

     

    Book Chapters

    Yi, L., and Liu, T. 2017. Engineering Microbial Metabolism for Chemical Synthesis. P97-120. In: Fatty acid biosynthesis and its metabolic engineering applications, Eds. Yajun Yan. World Scientific Press, USA.

    Liu, T. 2017. Microbiology. P150-171. In: Microbial genetics and breeding, Eds. Zixin Deng. Higher Education Press, China

    Ning, T., and Liu, T. 2014. Engineering Microbial Fatty Acid Biosynthesis Pathways to Make Advanced Biofuels. P197-218. In: Biofuels, from mircobes to moleculars, Eds. Xuefeng Lu. Caister Academic Press, U.K.

     Zhi, L., and Liu, T. 2013. New strategies for development of production of biofuels via the in vitro reconstitution of the fatty acid. Chapter 8, In: 2013 Industrial biotechnology development report. Eds. Yanhe Ma. Science Press, China

    Selected International and National Presentations

    1. Targeted Synthetic Metabolism for Overprodcution of Natral Products to Meet the Industrilization. (Invited talk, Feature speaker) 2018 Metabolic Engineering 12, German, 2018.

    2. Metabolic Engineering for Terpenoids Overproduction and Discovery. 2017 Metabolic Engineering Summit, China, 2017.

    3. Metabolic Engineering for Polyether Compound Overproduction and New Activities Discovery.18th International Symposium on the Biology of Actinomycetes, Korea, 2017.

    4. When Metabolic Engineering meets Natural Products Discovery. (Plenary talk) The 13th International Symposium on the Genetics of Industrial Microorganisms, China, 2016.

    5. In Vitro Reconstitution of Mevalonate Pathway Guide to Build the Terpenoids Over production Cell Factories. (Invited talk, Feature speaker) 2016 Metabolic Engineering 11, Japan, 2016

    6. In vitro Reconstitution Guide for Targeted Engineering, cell Factories & Biosustainability 2015, Denmark, 2015

    7. In vitro Reconstitution Guide for Targeted Engineering-Build for Understanding, Cold Spring Harbor Asia conference on Synthetic Biology, China, 2014 


    Publications

    1. Ma T, Shi B, Ye Z, et al. 2019. Lipid engineering combined with systematic metabolic engineering of Saccharomyces cerevisiae for high-yield production of lycopene[J]. Metabolic engineering.

    2. Abernathy, M. H., Zhang, Y., Hollinshead, W. D., Wang, G., Baidoo, E. E., Liu, T., & Tang, Y. J. 2019. Comparative studies of glycolytic pathways and channeling under in vitro and in vivo modes.

    3. Xiaojie Wang, Zixin Deng, Tiangang Liu*.2019. Marker-free system using ribosomal promoters enhanced xylose/glucose isomerase production in Streptomyces rubiginosus. biotechnology journal,(accepted)

    4. Yingying Chang, Baozhong Chai, Yunkun Ding, Min He, Linghui Zheng, Yun Teng, Zixin Deng, Yi Yu*, Tiangang Liu*.2019. Overproduction of gentamicin B in industrial strain Micromonospora echinospora CCTCC M 2018898 by cloning of the missing genes genR and genS. Metabolic engineering communications. (accepted)

    5. Yuan Y, Litzenburger M, Cheng S, Bian G, Hu B, Yan P, Cai Y, Deng Z, Bernhardt R, Liu T. 2018. Sesquiterpenoids Produced by the Combination of Two Sesquiterpene Cyclases with Promiscuous Myxobacterial CYP260B1. 

    6. Huang M, Liu B, Liu R, Li J, Chen J, Jian F, Ding H, Deng ZX, Liu T. 2018. Aglycone Polyether Nanchangmycin and Its Homologues Exhibit Apoptotic and Antiproliferative Activities against Cancer Stem Cells. 

    7. Bian G, Rinkel J, Wang Z, Lauterbach L, Hou A, Yuan Y, Deng Z, Liu T, Dickschat JS. 2018. A Clade II-D Fungal Chimeric Diterpene Synthase from Colletotrichum gloeosporioides Making Dolasta-1(15), 8-diene.

    8. Mary A, Zhang Y, Whitney H, Wang G, Baidoo E, Liu T, Tang Y. 2018. Comparative Studies of Glycolytic Pathways and Channeling Under in Vitro and in Vivo Modes. 

    9. Zhang, Y., Huang, Q., Deng, Z., Xu, Y., Liu, T*. (2018). Enhancing the efficiency of cell-free protein synthesis system by systematic titration of transcription and translation components. Biochemical Engineering Journal. https://doi.org/10.1016/j.bej.2018.07.001

    10. Bian, G., Ma, T., Liu, T*.  (2018). In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity. Methods in Enzymology.  https://www.sciencedirect.com/science/article/pii/S0076687918301769

    11. Tao, H., Zhang, Y., Deng, Z., Liu, T*. (2018). Strategies for Enhancing the Yield of the Potent Insecticide Spinosad in Actinomycetes. Biotechnology journal. 1700769. https://onlinelibrary.wiley.com/doi/epdf/10.1002/biot.201700769

    12. Liu, R., Deng, Z., Liu, T*. (2018). Streptomyces species: Ideal chassis for natural product discovery andoverproduction. Metabolic Engineering.   https://doi.org/10.1016/j.ymben.2018.05.015

    13. Ding, J., Liang, H., Fu, S., Liu, R., Deng, Z., Liu, T*. (2018). Modification of E-Poly-L-Lysine In Vivo to Reduce Self-Toxicity and Enhance Antibiotic Overproduction. AIChE Journal. DOI 10.1002/aic.16190.

    14. Bian, G., Hou, A., Yuan, Y., Hu, B., Cheng, S., Ye, Z., Di, Y., Deng, Z., Liu, T*. (2018). Metabolic engineering-based rapid characterization of a sesquiterpene cyclase and the skeletons of fusariumdiene and fusagramineol from Fusarium graminearum. Organic Letters. 20(6):1626-1629.

    15. Long, Y., Liu, R., Liang, H., Liu, T*. (2018). High-throughput screening method for high-yield nisin strain. Acta Microbiologica Sinica. 58(7): 1298-1308

    16. Liu, T*., Chu, Young, Kim. Editorial overview: Pharmaceutical biotechnology. (2017). Current Opinion in Biotechnology. 48: 258-259.

    17. Bian, G., Deng, Z., Liu, T*. (2017). Strategies for terpenoid overproduction and new terpenoid discovery. Current Opinion in Biotechnology. 48:234-241.

    18. Bian, G., Han, Y., Hou, A., Yuan, Y., Liu, X., Deng, Z., Liu, T*. (2017). Releasing the potential power of terpene synthases by a robust precursor supply platform. Metabolic Engineering. 42:1-8.

    19. Tan, G., Deng, K., Liu, X., Tao, H., Chang, Y., Chen, J., Chen, K., Sheng, Z., Deng, Z., Liu, T*. (2017). Heterologous Biosynthesis of Spinosad: An Omics-Guided Large Polyketide Synthase Gene Cluster Reconstitution in Streptomyces. ACS Synthetic Biology. 6(6):995-1005

    20. Bian, G., Yuan, Y., Tao, H., Shi. X., Zhong, X., Han, Y., Fu, S., Fang, C., Deng, Z., Liu, T*. (2017). Production of Taxadiene by engineering of mevalonate pathway in Escherichia coli and endophylic fungus Alternaria alternata TPF6. Biotechnology Journal. 12(4).

    21. Liu, Z., Zhang, Y., Jia, X., Hu, M., Deng, Z., Xu, Y., Liu, T*. (2017). In Vitro Reconstitution and Optimization of the Entire Pathway to Convert Glucose into Fatty Acid. ACS Synth Biol.  6(4):701-709.

    22. Xie, L., Ning, T., Bian, G., Gao, D., Liu, T*. (2017). Fatty acid insertion mechanism during membrane synthesis in Escherichia coli. Acta Microbiologica Sinica. 57(5): 769-780

    23. Huang, Q., Cao, X., Zhang, Y., Liu, T*., Xu, Y*. (2017). Application of a Novel Thioester Decomposition Reaction in Fatty Acid Biosynthesis Intermediates Analysis. Medical Journal of Wuhan University. 38(05) 41-44

    24. Hu, Y., Zhang, Y., Xu, Y*., Liu, T*. (2017). Synthesis of soluble proinsulin in a cell-free protein synthesis system. Chinese Journal of Biotechnology. 3:467-477

    25. Tan, G., Liu, T*. (2016). Rational synthetic pathway refactoring of natural products biosynthesis in actinobacteria. Metabolic Engineering. 6(6):995-1005.

    26. Liu, Q., Xiao, L., Zhou, Y., Deng, K., Tan, G., Han, Y., Liu, X., Deng, Z., Liu, T*. (2016). Development of Streptomyces sp. FR-008 as an Emerging Chassis. Synthetic and Systems Biotechnology. 1:207-214.

    27. Tan, G., Zhu, F., Deng, Z., Liu, T*. (2016). In vitro Reconstitution guide for targeted synthetic metabolism of chemicals, nutraceuticals and drug precursors. Synthetic and Systems Biotechnology. 1(1):25-33.

    28. Tao, H., Zhang, Y., Cao, X., Deng, Z., Liu, T*. (2016). Absolute Quantification of Proteins in the Fatty Acid Biosynthetic Pathway Protein Standard Absolute Quantification. Synthetic and Systems Biotechnology. 1(1):150-157

    29. Huang, M., Deng, Z., Tian, J., Liu, T*. (2016). Synthesis and biological evaluation of salinomycin triazole analogues as anticancer agents. European Journal of Medicinal Chemistry. http://dx.doi.org/10.1016/j.ejmech.2016.10.067

    30. Liu, Z., Liu, T*. (2016). Production of acrylic acid and propionic acid by constructing a portion of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula in Escherichia coli. J Ind Microbiol Biotechnol. 43:1659-1670.

    31. Hou, A., Deng, Z., Ma, H., Liu, T*. (2016). Substrate screening of amino transaminase for the synthesis of a sitagliptin intermediate. Tetrahedron. 72(31):4660-4664

    32. Ye, Z., Li, X., Cheng, Y., Liu, Z., Tan, G., Zhu, F., Fu, A., Deng, Z., Liu, T*. (2016). Evaluation of 3-hydroxypropionate biosynthesis in vitro by partial introduction of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula. J Ind Microbiol Biotechnol .43:1313-321.

    33. Ma, T., Deng, Z., Liu, T*. (2016). Microbial production strategies and applications of lycopene and other terpenoids. World Journal of Microbiology and Biotechnology. 32:15.

    34. Ma, T., Zhou, Y., Li, X., Zhu, F., Cheng, Y., Li. Y., Deng, Z., Liu, T*. (2016). Genome mining of astaxanthin biosynthetic genes from Sphingomonas sp. ATCC 55669 for heterologous overproduction in E. coli. Biotechnology Journal. 11:228–237

    35. Lu, J., Liu, Y., Guo, D., Deng, Z., Liu, T*. (2016). Production of fatty alcohol in Komagataella pastoris by heterologous expression of fatty acyl-CoA reductase. Microbiology China. 43(6): 1181-1189

    36. Jia, X., Liu, T*. (2016). Progress of cell-free protein synthesis system and its applications in pharmaceutical engineering. Acta Microbiologica Sinica. 56(3): 530-542

    37. Xiao, L., Liu, T*. (2016). Progress in developing and applying Streptomyces chassis. Acta Microbiologica Sinica. 56(3):441-453

    38. Tao, H., Guo, D., Zhang, Y., Deng, Z., Liu, T*. (2015). Metabolic engineering of microbes for branched-chain biodiesel production with low-temperature property. Biotechnology for Biofuels. 8:92.

    39. Tan, G., Deng, Z., Liu, T*. (2015). Recent advances in the elucidation of enzymatic function in natural product biosynthesis. F1000 Research.

    40. Zhu, F., Lu, L., Fu, S., Zhong, X., Hu, M., Deng, Z., Liu, T*. (2015). Targeted engineering and scale up of lycopene overproduction in Escherichia coli. Process Biochemistry. 50, 341-346.

    41. Liu, Q., Wu, K., Lu, L., Cheng, Y., Zhang, Y., Deng, Z., Liu, T*. (2015). Engineering an iterative polyketide pathway in Escherichia coli results in single-form alkene and alkane overproduction. Metabolic Engineering. 28, 82-90.

    42. Cheng, Y., Deng, Z., Liu, T*. (2015). Quantitative monitoring targeted proteins and intermediate metabolites in Escherichia coli primary metabolic pathways. Acta Microbiologica Sinica. 5511),1458-1467

    43. Liu, R., Zhu, F., Lu, L., Fu, A., Lu, J., Deng, Z., Liu, T*. (2014). Metabolic engineering of fatty acyl-ACP reductase-dependent pathway to improve fatty alcohol production in Escherichia coli. Metabolic engineering. 22, 10-21.

    44. Li, X., Guo, D., Cheng, Y., Zhu, F., Deng, Z., Liu, T*. (2014). Overproduction of fatty acids in engineered Saccharomyces cerevisiae. Biotechnology and bioengineering. 111(9):1841-52

    45. Guo, D., Zhu, J., Deng, Z., Liu, T*. (2014). Metabolic Engineering of Escherichia coli for Production of Fatty Acid Short-Chain Esters through Combination of the Fatty Acid and 2-Keto Acid Pathways. Metabolic engineering. 22, 69-75

    46. Wang, J., Nie, Y., Lu, Y., Liu, J., Wang, J., Fu, A., Liu, T*. Xia, J. (2014). Assembly of Multivalent Protein Ligands and Quantum Dots: A Multifaceted Investigation. Langmuir. 30, 2161–2169

    47. Zhu, F., Zhong, X., Hu, M., Lu, L., Deng, Z., Liu, T*. (2014). In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli. Biotechnology and bioengineering. 111, 1396-1405

    48. Wang, Y., Liu, Z., Liu, T*. (2013).Excavation and releasing the potential of fatty acid biosynthesis system. Chinese Bulletin of Life Sciences. 25(10), 966-77

    49. Yu, X., Liu, T*., Zhu, F., Khosla, C. (2011). In vitro reconstitution and steady-state analysis of the fatty acid synthase from Escherichia coli. Proc Natl Acad Sci U S A. 108(46):18643-8

    50. Fu, A., Liu, R., Zhu, J., Liu, T*. (2011). Genetic engineering of microbial metabolic pathway for production of advanced biodiesel. Hereditas. 33(10), 1121-1133

    51. Liu, T., Khosla C. (2010). A Balancing Act for Taxol Precursor Pathways in E. coli. Science. 330:44-45

    52. Liu, T., Vora H, Khosla C. (2010). Quantitative analysis and engineering of fatty acid biosynthesis in E. coli. Metabolic Engineering. 12:378-86

    53.  Liu, T., Khosla C. (2010). Genetic Engineering of Escherichia coli for Biofuel production. Annual Review of Genetics. 44:53-69

    54. Liu, T., Cane D, Deng Z. (2009).The Enzymology of Polyether Biosynthesis. Methods in Enzymology. 459:187-214

    55. Liu, T., Lin X, Zhou X, Deng Z, Cane D. (2008). Mechanism of thioesterase-catalyzed chain release in the biosynthesis of the polyether antibiotic nanchangmycin. Chemistry & Biology.15, 449-58

    56. Liu, T., You D, Valenzano C, Sun Y, Li J, Yu Q, Zhou X, Cane D, Deng Z. (2006). Identification of NanE as the Thioesterase for Polyether Chain Release in Nanchangmycin Biosynthesis. Chemistry & Biology. 13945–955